Chao T Y, Chu T M
Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1990 Dec;14(4):223-7.
The in vivo distribution of intravenously injected lymphokine activated killer (LAK) cells, generated in vitro with rIL-2 from normal murine splenocytes, was studied in BALB/c mice and compared with that of normal splenocytes. Both normal splenocytes and LAK cells were labeled with 51Cr, and the results were analyzed at 6, 24, and 48 hours after injection by localization index as the parameter. After injection through tail veins of mice, LAK cells were found to migrate to the spleen, lungs, liver, lymph nodes, bones and the kidneys. The apparent increased distribution pattern of LAK cells to the lung at 6 and 24 hours after injection was not detected when normal splenocytes were injected. Since almost one third of the injected LAK cells were found to localize in the spleen, it was postulated that splenectomy would affect the in vivo organ distribution of LAK cells. Accordingly, the in vivo distribution of LAK cells in splenectomized mice was further investigated. Results indicated that splenectomy enhanced the convergence of LAK cells to the lungs, liver, lymph nodes and bones. Therefore, splenectomy may augment the therapeutic effect of the adoptive transfer of LAK cells in pulmonary, hepatic, lymph node and bony metastases.
研究了用重组白细胞介素-2（rIL-2）体外激活正常小鼠脾细胞产生的静脉注射淋巴因子激活的杀伤细胞（LAK细胞）在BALB/c小鼠体内的分布，并与正常脾细胞的分布进行了比较。正常脾细胞和LAK细胞均用51Cr标记，以定位指数为参数，在注射后6小时、24小时和48小时分析结果。经小鼠尾静脉注射后，发现LAK细胞迁移至脾脏、肺、肝脏、淋巴结、骨骼和肾脏。注射正常脾细胞时，未检测到注射后6小时和24小时LAK细胞在肺中的明显增加的分布模式。由于发现几乎三分之一的注射LAK细胞定位于脾脏，因此推测脾切除术会影响LAK细胞在体内的器官分布。据此，进一步研究了脾切除小鼠体内LAK细胞的分布。结果表明，脾切除术增强了LAK细胞向肺、肝脏、淋巴结和骨骼的聚集。因此，脾切除术可能会增强LAK细胞过继性转移对肺、肝、淋巴结和骨转移的治疗效果。
