Negishi K, Yamashita T, Zacharias W, Wells R D, Bessho T, Hayatsu H
Gene Research Center, Okayama University, Japan.
Nucleic Acids Symp Ser. 1990(22):109-10.
Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions.
用亚硫酸氢盐 - 甲氧基胺（一种对单链核酸具有特异性的修饰试剂）探测了pWR756（一种含有(GC)16的质粒）中盐诱导和超螺旋诱导的B - Z连接。用S1核酸酶分析修饰位点，并根据DNA聚合酶进行DNA链延伸的终止位点确定修饰的胞嘧啶。结果表明，两种类型的B - Z连接中，大多数可及的胞嘧啶是相同的。
