Negishi K, Yamashita T, Zacharias W, Wells R D, Bessho T, Hayatsu H
Gene Research Center, Okayama University, Japan.
Nucleic Acids Symp Ser. 1990(22):109-10.
Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions.
Zotero 插件