氨酰-tRNA合成酶对tRNA识别决定因素的识别。
Recognition of tRNA identity determinants by aminoacyl-tRNA synthetases.
作者信息
Muramatsu T, Nureki O, Kanno H, Niimi T, Tateno M, Kohno T, Kawai G, Miyazawa T, Muto Y, Yokoyama S
机构信息
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser. 1990(22):119-20.
By analyzing aminoacylation activities of variants of isoleucine tRNAs and glutamic acid tRNA from Escherichia coli, it was found that the anticodons are the major determinants for "identities" of these tRNAs. It was also shown that the post-transcriptional modifications are essential to aminoacylation of these tRNAs. Interactions between the tRNAs and the aminoacyl-tRNA synthetases were analyzed by NMR spectroscopy.
通过分析来自大肠杆菌的异亮氨酸tRNA和谷氨酸tRNA变体的氨酰化活性，发现反密码子是这些tRNA“身份”的主要决定因素。还表明转录后修饰对于这些tRNA的氨酰化至关重要。通过核磁共振光谱分析了tRNA与氨酰-tRNA合成酶之间的相互作用。
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