Komatsu H, Ichikawa T, Takaku H, Yokoyama S, Kawai G
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Nucleic Acids Symp Ser. 1990(22):95-6.
The modified base and sugar moieties, 8-oxo-7,8-dihydroadenine (HO8A),8-methoxyadenine (MeO8A),8-methoxyguanine (MeO8G) and 9-[(2-hydroxy-1-(hydroxymethyl)-ethoxy)-methyl]-adenine (acA), were chemically introduced in place of the adenine or guanine in the octa-deoxyribonucleotides [d(GGAATTCC)] containing recognition sequence of Eco RI. to the regular DNA-fragment.
将修饰的碱基和糖部分，即8-氧代-7,8-二氢腺嘌呤（HO8A）、8-甲氧基腺嘌呤（MeO8A）、8-甲氧基鸟嘌呤（MeO8G）和9-[(2-羟基-1-(羟甲基)-乙氧基)-甲基]-腺嘌呤（acA），化学引入到含有Eco RI识别序列的八聚脱氧核糖核苷酸[d(GGAATTCC)]中，以取代腺嘌呤或鸟嘌呤，从而得到常规的DNA片段。
