采用双电极安培检测器的液相色谱法检测尿液中的N-乙酰半胱氨酸、半胱氨酸及其二硫化物。
Detection of N-acetylcysteine, cysteine and their disulfides in urine by liquid chromatography with a dual-electrode amperometric detector.
作者信息
Stenken J A, Puckett D L, Lunte S M, Lunte C E
机构信息
Department of Chemistry, University of Kansas, Lawrence 66045.
出版信息
J Pharm Biomed Anal. 1990;8(1):85-9. doi: 10.1016/0731-7085(90)80011-d.
A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually.
本文描述了一种测定尿液中N-乙酰半胱氨酸、半胱氨酸及其二硫化物的方法。硫醇和二硫化物通过以硫酸辛酯钠为离子对试剂的反相离子对色谱法进行分离，并用金/汞齐电极的双电极安培检测器进行检测。该系统可同时检测硫醇和二硫化物。此外，二聚体和混合二硫化物也可单独检测。
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