Hidayat A, Hibbert D B, Alexander P W
Department of Analytical Chemistry, University of New South Wales, Sydney, Australia.
J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):139-46. doi: 10.1016/s0378-4347(97)00054-6.
The amperometric detection of thiols following their separation by reversed-phase chromatography and reaction into a post-column mercury carrier stream is shown to be a sensitive method when using a tungsten wire sensor as the working electrode in a three-electrode flow cell. Five organic thiols (cysteine, homocysteine, reduced glutathione, D,L-penicillamine and 2-mercaptopropionic acid) and thiourea could be separated within approx. 18 min. The analytical performance is comparable, and stability superior, to chemically modified electrodes previously reported.
通过反相色谱分离硫醇并使其与柱后汞载流反应后，采用钨丝传感器作为三电极流通池中的工作电极进行安培检测，结果表明这是一种灵敏的方法。五种有机硫醇（半胱氨酸、同型半胱氨酸、还原型谷胱甘肽、D,L-青霉胺和2-巯基丙酸）和硫脲可在约18分钟内分离。其分析性能与先前报道的化学修饰电极相当，稳定性更佳。
