Ebert P R, Clarke A E
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria.
Plant Mol Biol. 1990 May;14(5):815-24. doi: 10.1007/BF00016514.
A transformation and regeneration system has been developed for Nicotiana alata, a plant which is being intensively studied as a model of gametophytic self-incompatibility. Plantlets can be regenerated efficiently from seedling hypocotyls. Kanamycin-resistant, transformed plants have been obtained by cocultivation of regenerating hypocotyls with Agrobacterium tumefaciens strain LBA4404 containing a binary vector. The transformation frequency was low with less than 1% of tissue explants regenerating transformed plants. The transformed plants contained from one to three copies of the introduced DNA. In most cases, the kanamycin resistance phenotype was transmitted to the offspring as a normal Mendelian factor. In one unusual case, none of the offspring inherited the kanamycin resistance of the transformed maternal parent. This plant may have been chimeric or the kanamycin resistance gene may have been inactivated.
已为烟草（Nicotiana alata）开发了一种转化和再生系统，烟草作为配子体自交不亲和模型正受到深入研究。植株可从幼苗下胚轴高效再生。通过将再生下胚轴与含有二元载体的根癌农杆菌（Agrobacterium tumefaciens）菌株LBA4404共培养，获得了抗卡那霉素的转化植株。转化频率较低，再生转化植株的组织外植体不到1%。转化植株含有1至3个导入DNA拷贝。在大多数情况下，卡那霉素抗性表型作为正常孟德尔因子传递给后代。在一个不寻常的案例中，没有一个后代继承转化母本的卡那霉素抗性。该植株可能是嵌合体，或者卡那霉素抗性基因可能已失活。
