Department of Physics and Optical Science, University of North Carolina Charlotte, Charlotte, North Carolina, USA.
J Phys Chem B. 2010 Nov 25;114(46):15134-40. doi: 10.1021/jp1032146. Epub 2010 Oct 28.
Two 25 base-pair cDNA strands are encapsulated within an optically trapped nanodroplet, and we observe the kinetics of their hybridization in dynamic equilibrium via single-molecule fluorescence resonance energy transfer (FRET) as a function of temperature and of the solution's NaCl concentration. We have observed the duplex unfolding and refolding, and we have observed quasistable partially unfolded states under low salinity conditions. Furthermore, our measurements reveal that, even in conditions under which the duplex is stable, it undergoes conformational fluctuations in solution.
两段 25 个碱基对的 cDNA 链被包裹在一个光阱纳米液滴中，我们通过单分子荧光共振能量转移（FRET）观察它们在动态平衡中的杂交动力学，作为温度和溶液 NaCl 浓度的函数。我们已经观察到双链体的展开和重折叠，并且在低盐条件下观察到准稳定的部分展开状态。此外，我们的测量结果表明，即使在双链体稳定的条件下，它在溶液中也会经历构象波动。
