Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, Turkey.
Clin Exp Hypertens. 2010;32(7):458-63. doi: 10.3109/10641961003686468.
Our objective was to investigate the endothelial nitric oxide synthase (eNOS) immuno-reactivity and the ultrastructure of endothelial cells of a human umbilical artery in both normal and preeclamptic pregnancies. The umbilical cords from normal and preeclamptic pregnancies were collected immediately after vaginal and abdominal deliveries. Umbilical arteries were isolated and fixed in 10% neutral formaline solution, embedded in paraffin, and then stained with hematoxylin and eosin (H&E) for the histologic investigation, and eNOS activation were examined in samples by streptavidine-biotine immunohistochemical methods. The arterial sections were also fixed in phosphate-buffered 2.5% glutaraldehyde solution (pH 7.2) for 3 h and post-fixed with 1% osmium tetroxide at 4°C for 2 h for the investigation of the ultrastructural examination. In the umbilical artery of preeclamptic pregnancies, endothelial cells were oval, triangular, or polygonal, and were disorganized. Some endothelial cells were separated by enlarged intercellular spaces. A dilated endoplasmic reticulum, swollen mitochondria, and vanished mitochondrial cristae were observed. The nuclei of some endothelial cells displayed deep invaginations and irregular outlines. Most endothelial cells had a high number of cytoplasmic vacuoles. In preeclampsia, eNOS immunoreactivity increased considerably in endothelial cells when compared to normal pregnancies. We believe that preeclampsia plays an important role in the pathogenesis of endothelial cell dysfunction and activation in the umbilical artery. However, the disturbance mechanism of endothelial cells is not known, and further studies are necessary to clarify the exact mechanism.
我们的目的是研究正常和子痫前期妊娠的人脐带动脉内皮型一氧化氮合酶（eNOS）免疫反应性和内皮细胞的超微结构。正常和子痫前期妊娠的脐带在阴道和腹部分娩后立即收集。将脐动脉分离并固定在 10％中性福尔马林溶液中，嵌入石蜡中，然后用苏木精和伊红（H＆E）进行组织学研究，并通过链霉亲和素-生物素免疫组织化学方法检查样本中的 eNOS 激活。动脉切片也在磷酸盐缓冲的 2.5％戊二醛溶液（pH 7.2）中固定 3 小时，并在 4°C 下用 1％锇四氧化物后固定 2 小时，用于超微结构检查。在子痫前期的脐动脉中，内皮细胞呈椭圆形，三角形或多边形，排列紊乱。一些内皮细胞被扩大的细胞间隙隔开。观察到扩张的内质网，肿胀的线粒体和消失的线粒体嵴。一些内皮细胞核显示出深的内陷和不规则的轮廓。大多数内皮细胞具有大量的细胞质空泡。与正常妊娠相比，子痫前期中内皮细胞的 eNOS 免疫反应性显著增加。我们认为子痫前期在脐动脉内皮细胞功能障碍和激活的发病机制中起重要作用。然而，内皮细胞的干扰机制尚不清楚，需要进一步研究以阐明确切的机制。
