删除马立克氏病病毒 1.8kb mRNA 会降低其复制能力但不会降低其致癌性。
Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity.
机构信息
Animal Science and Technology College, Shandong Agricultural University, Tai'an, Shandong 271018, China.
出版信息
Virol J. 2010 Oct 29;7:294. doi: 10.1186/1743-422X-7-294.
BACKGROUND
The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA.
RESULTS
Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101Δ(A+C) was constructed. The present experiments indicated that GX0101Δ(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV.
CONCLUSION
These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV.
背景
1.8kb mRNA 被报道为马立克氏病病毒（MDV）致癌相关基因之一。在本研究中，使用 MDV 田间株 GX0101 的细菌人工染色体（BAC）克隆作为平台，生成突变型 MDV，以研究 1.8kb mRNA 的功能作用。
结果
基于 GX0101 的 BAC 克隆，构建了 1.8kb mRNA 缺失突变株 GX0101Δ(A+C)。本实验表明，与亲本病毒 GX0101 相比，GX0101Δ(A+C)保留了低水平的致癌性，并且在体外和体内的复制能力均降低。进一步的体外研究表明，1.8kb mRNA 的缺失显著降低了 MDV 1.8kb mRNA 和 pp38 基因之间双向启动子的转录活性。
结论
这些结果表明，1.8kb mRNA 并不直接影响肿瘤发生，而是与 MDV 的复制能力有关。
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