Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland.
Bioresour Technol. 2011 Feb;102(3):3028-33. doi: 10.1016/j.biortech.2010.09.124. Epub 2010 Oct 8.
Three Pseudomonas aeruginosa strains isolated from petroleum-polluted soil were the subject of studies concerning changes in cell surface properties. Fundamentally different reactions could be observed for each of the studied strains after a cultivation on various carbon sources. The experiments carried out during the logarithmic growth phase showed, that the changes in the cell surface hydrophobocity values were dynamic and substrate dependant. An external addition of rhamnolipids to the tested systems resulted in further shifts in the CSH values. All of the strains displayed miscellaneous phenotypic properties during MATH, sedimentation profile, Zeta potential and surface tension measurements. The obtained results lead to a conclusion, that the presence of rhamnolipids seems to be the key factor to this phenomenon, as all of the studied strains exhibited the ability to produce this biosurfactant in a different degree.
从石油污染土壤中分离出的三株铜绿假单胞菌菌株是研究细胞表面特性变化的对象。在对各种碳源进行培养后，每种研究菌株的反应都截然不同。在对数生长期进行的实验表明，细胞表面疏水性值的变化是动态的且取决于基质。向测试系统中添加鼠李糖脂会导致 CSH 值进一步变化。在 MATH、沉降剖面、Zeta 电位和表面张力测量过程中，所有菌株均表现出多种表型特性。所得结果得出结论，鼠李糖脂的存在似乎是该现象的关键因素，因为所有研究菌株均表现出不同程度产生该生物表面活性剂的能力。
