镰刀菌属对 4-氯苯酚生物降解的特性和酶活性
The characteristics and enzyme activities of 4-chlorophenol biodegradation by Fusarium sp.
机构信息
College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, China.
出版信息
Bioresour Technol. 2011 Feb;102(3):2985-9. doi: 10.1016/j.biortech.2010.10.006. Epub 2010 Oct 8.
The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1 g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis-Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01.
考察了 pH 值、温度和蔗糖添加对真菌 HJ01 降解 4-氯苯酚（4-CP）的生物降解能力的影响，研究了真菌 HJ01 在降解 4-CP 过程中产生的双加氧酶的特性。结果表明，真菌 HJ01 对溶液中的 4-CP 具有很高的降解能力。最适 pH 值、蔗糖浓度和温度分别为 pH7、1g/L 和 30°C。该菌株可以产生邻氯儿茶酚 1,2-双加氧酶（CC12O）和邻氯儿茶酚 2,3-双加氧酶（CC23O），当以 4-CP 作为唯一碳源和能源时，其活性最高，CC12O 的最适 pH 值和温度分别为 pH7 和 50°C，CC23O 的最适 pH 值和温度分别为 pH8 和 60°C。酶催化反应的动力学符合米氏方程。据我们所知，这是首次研究真菌 HJ01 对 4-CP 的生物降解作用。
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