4Pi 显微镜中的偏振效应。

Polarization effects in 4Pi microscopy.

机构信息

Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, Singapore 117456, Singapore.

Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore.

出版信息

Micron. 2011 Jun;42(4):353-9. doi: 10.1016/j.micron.2010.07.013. Epub 2010 Sep 18.

DOI:10.1016/j.micron.2010.07.013

PMID:21030264

Abstract

The effects of different apodization conditions and polarization distributions on imaging in 4Pi microscopy are discussed. Performance parameters are derived that allow the different implementations to be compared. 4Pi microscopy is mainly used because of its superior axial imaging performance, but it is shown that transverse resolution is also improved in the 4Pi geometry, by as much as 25% compared with focusing by a single aplanatic lens. Compared with plane-polarized illumination in a 4Pi aplanatic system, transverse resolution in the 4Pi mode can also be increased by about 18%, using radially polarized illumination, but at the expense of axial resolution. The electric energy density at the focus for a given power input can be increased using electric dipole polarization, which is relevant for atomic physics experiments such as laser trapping and cooling.

摘要

讨论了不同的消光条件和偏振分布对 4Pi 显微镜成像的影响。得出了性能参数，允许比较不同的实现。4Pi 显微镜主要因其优越的轴向成像性能而被使用，但也表明在 4Pi 几何中，横向分辨率也得到了改善，与单个消色差透镜聚焦相比，改善了 25%。与平面偏振照明在 4Pi 消色差系统中相比，使用径向偏振照明，4Pi 模式中的横向分辨率也可以提高约 18%，但代价是轴向分辨率。对于给定的功率输入，使用电偶极子偏振可以增加焦点处的电能密度，这对于激光俘获和冷却等原子物理实验是相关的。