Very P, Bocquentin R, Duvallet G
Centre de Recherches sur les Trypanosomoses Animales, Burkina Faso.
Rev Elev Med Vet Pays Trop. 1990;43(3):325-9.
The double microcentrifugation technique, described by KRATZER and ONDIEK (1989) for the parasitological diagnosis of trypanosomes, has been tested both in the laboratory and in the field. The limits of detection obtained here were not as low as those described in the original experiment, but the sensitivity of this technique for the detection of Trypanosoma brucei, T. congolense and T. vivax was better than the phase contrast buffy coat method. This technique, which is easy to apply in the field, is highly recommended, especially for epidemiological surveys. A protocol and a list of equipment are included.
克拉策尔和翁迪克（1989年）描述的用于锥虫寄生虫学诊断的双重微量离心技术，已在实验室和现场进行了测试。这里获得的检测限不如原始实验中描述的那么低，但该技术对布氏锥虫、刚果锥虫和活泼锥虫的检测灵敏度优于相差血沉棕黄层法。这种技术易于在现场应用，强烈推荐使用，特别是用于流行病学调查。文中包括了一份操作流程和一份设备清单。
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