Wiwatanaratanabutr Itsanun, Allan Sandra, Linthicum Kenneth, Kittayapong Pattamaporn
Center of Excellence for Vectors and Vector-Borne Diseases and Department of Biology, Faculty of Science, Mahidol University at Salaya, Nakhon Pathom 73170, Thailand.
J Am Mosq Control Assoc. 2010 Sep;26(3):265-73. doi: 10.2987/09-5937.1.
Wolbachia are maternally inherited bacteria that cause various reproductive alterations in their arthropod hosts, including cytoplasmic incompatibility. In this study, we compared mating, oviposition, and host-seeking behavior of Wolbachia-infected (Houston [HOU], Gainesville [GNV]) and Houston uninfected (HT1) Aedes albopictus. In mating assays with virgin mosquitoes, mating success of Wolbachia-infected males was significantly higher than uninfected strains. Mating success was highest with HOU males exposed to infected (95%) and uninfected females (100%), and lowest with HT1 males exposed to infected (20%) and uninfected (25%) females. Results suggested that Wolbachia infection may influence the reproductive behavior of this mosquito. There were no clear differences in oviposition responses between strains, with all strains ovipositing significantly more often on hay infusion and larval rearing water than on water controls and least frequently on 4-methylphenol. Strains of Ae. albopictus females were host-seeking a human when given a choice. Responses to a human arm, acetone, CO2, and dichloromethane were generally higher from the Houston strains than from the GNV strain. Responses of HOU and HT1 females differed from GNV with greater responses to the arm and CO2.
沃尔巴克氏体是通过母体遗传的细菌，会在其节肢动物宿主中引发各种生殖改变，包括细胞质不亲和性。在本研究中，我们比较了感染沃尔巴克氏体的白纹伊蚊（休斯顿株[HOU]、盖恩斯维尔株[GNV]）和未感染的休斯顿白纹伊蚊（HT1）的交配、产卵及宿主寻找行为。在与未交配过的蚊子进行的交配试验中，感染沃尔巴克氏体的雄蚊交配成功率显著高于未感染的品系。HOU雄蚊与感染雌蚊（95%）和未感染雌蚊（100%）交配时成功率最高，而HT1雄蚊与感染雌蚊（20%）和未感染雌蚊（25%）交配时成功率最低。结果表明，沃尔巴克氏体感染可能会影响这种蚊子的生殖行为。各品系在产卵反应上没有明显差异，所有品系在干草浸液和幼虫饲养用水上产卵的频率显著高于用水对照，而在4 - 甲基苯酚上产卵频率最低。当有选择时，白纹伊蚊雌蚊会寻找人类作为宿主。休斯顿品系对白纹伊蚊雌蚊对人手臂、丙酮、二氧化碳和二氯甲烷的反应通常高于盖恩斯维尔品系。HOU和HT1雌蚊的反应与GNV不同，对人手臂和二氧化碳的反应更强。
