[莱姆病患者中伯氏疏螺旋体基因菌株的分析]
[Analysis of Borrelia burgdorferi genostrains among patients with Lyme disease].
作者信息
Biesiada Grazyna, Czepiel Jacek, Salamon Dominika, Garlicki Aleksander, Dziubek Anna, Maziarz Barbara, Mach Tomasz
机构信息
Klinika Chorób Zakaźnych, Katedry Gastroenterologii, Hepatologii i Chorób Zakaźnych, Collegium Medicum, Uniwersytetu Jagiellońskiego w Krakowie.
出版信息
Przegl Lek. 2009;66(9):511-2.
BACKGROUND
Lyme borreliosis is a zoonose which can be transmitted to the humans during Ixodes tick bite to the skin. The disease is caused by bacteria--spirochetes of the Borrelia species, classified as Borrelia burgdorferi strain. In Poland Lyme borreliosis is caused by Borrelia garinii, Borrelia afzelii and Borrelia burgdorferi sensu stricto. The presence of Borrelia spielmani was recently reported.
MATERIAL AND METHODS
The study included 249 patients diagnozed in University Hospital in Krakow due to suspicion of Lyme borreliosis in 2005-2008. Results of serologic tests against borreliosis were analized using Elisa tests (Mikrogen), positive tests were confirmed using Western blot tests (Biomedica).
RESULTS
It was found the high percentage of the antibodies in class IgM, which are important in acute phase of borreliosis, typical for Borrelia garinii, whereas in class IgG it was found the prevalence of protein typical for Borrelia afzellii and Borrelia burgdorferi sensu stricto. It was found the high prevalence of the presence VIsE reacting with IgG antibodies. It was noted the presence of Borrelia spielmani genostrain in Poland.
背景
莱姆病是一种人畜共患病，可在硬蜱叮咬皮肤时传播给人类。该疾病由细菌——博氏疏螺旋体属的螺旋体引起，归类为伯氏疏螺旋体菌株。在波兰，莱姆病由伽氏疏螺旋体、阿氏疏螺旋体和狭义伯氏疏螺旋体引起。最近有报道称存在斯氏疏螺旋体。
材料与方法
该研究纳入了2005年至2008年在克拉科夫大学医院因疑似莱姆病而确诊的249例患者。使用酶联免疫吸附测定（ELISA）试验（Mikrogen）分析针对莱姆病的血清学检测结果，阳性检测结果通过蛋白质印迹试验（Biomedica）进行确认。
结果
发现IgM类抗体比例较高，这在莱姆病急性期很重要，是伽氏疏螺旋体的典型特征，而在IgG类中，发现阿氏疏螺旋体和狭义伯氏疏螺旋体典型的蛋白质占优势。发现与IgG抗体反应的VIsE存在率很高。注意到波兰存在斯氏疏螺旋体基因菌株。
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