McKnight M E, Koda K, DeBoer K, Glassy M C
Brunswick Biotechnetics, San Diego, California 92121.
Hum Antibodies Hybridomas. 1990;1(2):77-82.
Human lymph node lymphocytes from cancer patients were fused with either the UC 729-6 or SHFP-1 human fusion partners. Resulting human-human hybridomas were tetraploid, expressed markers from both parent cells, and secreted approximately 1 microgram Ig/10(6) cells/ml/day. Immunofluorescence analysis of some of the human MAbs with a panel of normal and malignant cell lines revealed a staining pattern of only the nuclear region. One IgM secreting hybridoma, TLN1F4, derived from a teratocarcinoma lymph node, predominantly stained the nuclear regions of adherent tumor cell lines and no hematopoietic cell lines or normal fibroblasts. PLN3C8, an IgG1 secreting hybridoma, derived from a prostate carcinoma lymph node, predominantly stained the nucleolus of LnCap, a a carcinoma of the prostate cell line. CLN2E5, an IgM secreting human hybridoma, derived from a carcinoma of the cervix lymph node, predominantly stained both cytoplasmic and nuclear components to tumor cell lines and not normal fibroblasts or hematopoietic cell lines. These data suggest that the immune response occurring within regional draining lymph nodes is capable of recognizing nuclear-associated antigens.
来自癌症患者的人淋巴结淋巴细胞与UC 729-6或SHFP-1人融合伙伴进行融合。产生的人-人杂交瘤是四倍体，表达来自两个亲本细胞的标志物，并分泌约1微克Ig/10(6)细胞/毫升/天。用一组正常和恶性细胞系对一些人单克隆抗体进行免疫荧光分析，结果显示仅核区域有染色模式。一种分泌IgM的杂交瘤TLN1F4，源自畸胎癌淋巴结，主要染色贴壁肿瘤细胞系的核区域，而不染色造血细胞系或正常成纤维细胞。PLN3C8是一种分泌IgG1的杂交瘤，源自前列腺癌淋巴结，主要染色前列腺癌细胞系LnCap的核仁。CLN2E5是一种分泌IgM的人杂交瘤，源自宫颈癌淋巴结，主要染色肿瘤细胞系的细胞质和核成分，而不染色正常成纤维细胞或造血细胞系。这些数据表明，区域引流淋巴结内发生的免疫反应能够识别与核相关的抗原。
