Casella S, Giannetto C, Giudice E, Piccione G
Department of Experimental Science and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, 98168 Messina, Italy.
Pol J Vet Sci. 2010;13(3):501-6.
The objective of the present study was to evaluate the platelet response to different workload in athletic horse and the influence of hydrocortisone on this response. For this purpose, six Sella Italiana gelding athletic horses, ranging in age from 8 to 10 years and clinically healthy, were subjected to four different exercises: horse-walker, treadmill, obstacle course of 1.00 m and obstacle course of 1.25 m. From all the horses, citrated plasma samples were collected at rest, immediately after exercise and 30 min after the end of the exercise to assess the maximum degree of platelet aggregation and the initial velocity of aggregation (slope) after platelet activation with 10 microM ADP and 20 microM ADP. Successively, the effect of 20 minutes pre-incubation with hydrocortisone in ADP-induced aggregation was determined. One-way repeated measures analysis of variance (ANOVA) was applied to determine significant differences due to experimental conditions (P < 0.05) and, for each sampling time, one-way analysis of variance was applied to determine significant differences due to workload (P < 0.05). Bonferroni's test was applied for post-hoc comparison. Our results revealed a statistically significant effect of experimental conditions only after obstacle course of 1.00 m and 1.25 m. These findings showed an inhibition of platelet aggregation during different workload in athletic horses that probably represents a protective endothelial mechanism through the production of nitrix oxide, and a kinetic measurement of the reaction that changes in relation to percentage of aggregation. The mechanism of hydrocortisone had no effect on platelet receptor expression in vitro in athletic horses, so it might be useful to evaluate the effect of different anti-inflammatory steroids in order to confirm, or note any differences, with drugs that have the same mechanism of action.
本研究的目的是评估运动马匹对不同工作量的血小板反应以及氢化可的松对该反应的影响。为此，选用了6匹8至10岁、临床健康的意大利塞尔拉种公马，使其进行4种不同的运动：马用步行器运动、跑步机运动、1.00米障碍赛和1.25米障碍赛。在所有马匹休息时、运动结束后即刻以及运动结束后30分钟采集枸橼酸盐血浆样本，以评估用10微摩尔/升二磷酸腺苷（ADP）和20微摩尔/升ADP激活血小板后血小板聚集的最大程度和聚集的初始速度（斜率）。随后，测定了氢化可的松预孵育20分钟对ADP诱导聚集的影响。采用单向重复测量方差分析（ANOVA）来确定由于实验条件导致的显著差异（P<0.05），并且对于每个采样时间，采用单向方差分析来确定由于工作量导致的显著差异（P<0.05）。采用Bonferroni检验进行事后比较。我们的结果显示，仅在1.00米和1.25米障碍赛后实验条件具有统计学显著效应。这些发现表明，运动马匹在不同工作量期间血小板聚集受到抑制，这可能代表了一种通过一氧化氮产生的保护性内皮机制，以及与聚集百分比相关的反应动力学测量。氢化可的松的作用机制在体外对运动马匹的血小板受体表达没有影响，因此评估不同抗炎类固醇的作用可能有助于确认或指出与具有相同作用机制的药物之间的任何差异。
