使用由周期性极化铌酸锂倍频分布反馈激光器驱动的便携式回音壁模式生物传感器进行超灵敏纳米颗粒检测。

Ultrasensitive nanoparticle detection using a portable whispering gallery mode biosensor driven by a periodically poled lithium-niobate frequency doubled distributed feedback laser.

作者信息

Shopova S I, Rajmangal R, Nishida Y, Arnold S

机构信息

MicroParticle PhotoPhysics Laboratory for BioPhotonics, Polytechnic Institute of New York University, Brooklyn, New York 11201, USA.

出版信息

Rev Sci Instrum. 2010 Oct;81(10):103110. doi: 10.1063/1.3499261.

DOI:10.1063/1.3499261

PMID:21034078

Abstract

We demonstrate a significant reduction in the limit of label-free detection of individual viral-sized nanoparticles in aqueous solution through the use of a frequency doubled telecom laser constructed from a distributed feedback-periodically poled lithium-niobate (DFB-PPLN) union. By driving a whispering gallery mode biosensor near a wavelength of 650 nm with this device we have detected real-time adsorption steps for particles 36 nm in radius with a signal to noise ratio of 8. The noise equivalent detection limit is ∼20 ag (17 nm radius). This new lower limit is attributed to the ultralow resonance wavelength noise [(Δλ(r))(rms)/λ(r)<10(-9)] associated with the use of the DFB-PPLN device.

摘要

我们展示了通过使用由分布反馈周期性极化铌酸锂（DFB-PPLN）组合构建的倍频电信激光器，水溶液中单个病毒大小的纳米颗粒的无标记检测限显著降低。通过用该装置在650nm波长附近驱动回音壁模式生物传感器，我们检测到了半径为36nm的颗粒的实时吸附步骤，信噪比为8。噪声等效检测限约为20 ag（半径17nm）。这个新的下限归因于与DFB-PPLN装置的使用相关的超低共振波长噪声[（Δλ(r))(rms)/λ(r)<10(-9)]。