Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
Dev Comp Immunol. 2011 Mar;35(3):345-51. doi: 10.1016/j.dci.2010.10.008. Epub 2010 Nov 17.
The chemokine RANTES plays an essential role in inflammation and immune response. In this study, we cloned the nucleotide sequence of the 5'-flanking region of the porcine RANTES (poRANTES) gene and characterized the regulatory elements that activate transcription. Analyses of a series of 5' deletion constructs demonstrated that a 266 bp region (-220/+46) that spanned the potential transcription start site of the poRANTES gene was sufficient to activate transcription in PK-15 cells. Furthermore, our results indicated that dsDNA/dsRNA significantly induced poRANTES promoter activity and expression of mRNA levels in a time- and dose-dependent manner. Promoter deletions and mutagenesis experiments indicated that an interferon-stimulated responsive element (ISRE) was critical for dsDNA/dsRNA-induced poRANTES transcription. In addition, porcine interferon regulatory factor 3 (IRF-3) and IRF-7 play important roles in dsDNA/dsRNA-induced poRANTES expression.
趋化因子 RANTES 在炎症和免疫反应中起着至关重要的作用。在这项研究中，我们克隆了猪 RANTES（poRANTES）基因 5'-侧翼区的核苷酸序列，并对激活转录的调节元件进行了特征分析。一系列 5'缺失构建体的分析表明，跨越 poRANTES 基因潜在转录起始位点的 266bp 区域（-220/+46）足以在 PK-15 细胞中激活转录。此外，我们的结果表明，dsDNA/dsRNA 以时间和剂量依赖的方式显著诱导 poRANTES 启动子活性和 mRNA 水平的表达。启动子缺失和突变实验表明，干扰素刺激反应元件（ISRE）对于 dsDNA/dsRNA 诱导的 poRANTES 转录至关重要。此外，猪干扰素调节因子 3（IRF-3）和 IRF-7 在 dsDNA/dsRNA 诱导的 poRANTES 表达中发挥重要作用。
