State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China.
Talanta. 2010 Nov 15;83(1):42-7. doi: 10.1016/j.talanta.2010.08.036. Epub 2010 Sep 29.
In this study, a highly selective, label-free electrochemical immunoassay strategy based on the charge transport through the multilayer films associated with the electrocatalytic reduction of Fe(CN)(6) is proposed using human immunoglobulin G (human IgG) as the model analyte. The antibody-antigen complex formed on the sensing interface can efficiently induce change of the surface charge characteristics, the conductivity of multilayer film and/or electron transfer distance, resulting in an immunoreaction signal. The current reduction is proportional to the amount of analyte. Under the optimized experimental conditions, the proposed sensing strategy provides a linear dynamic range from 10 to 10(4) ng mL(-1) and a detection limit of 3 ng mL(-1), indicating an improved analytical performance. This possibly makes it a potential alternative in bioanalysis of proteins and other molecules.
在这项研究中，提出了一种基于多层膜中电荷传递的高度选择性、无标记的电化学免疫分析策略，该策略与Fe(CN)(6)的电催化还原有关，用人免疫球蛋白 G（人 IgG）作为模型分析物。在传感界面上形成的抗体-抗原复合物可以有效地诱导表面电荷特性、多层膜的电导率和/或电子转移距离的变化，从而产生免疫反应信号。电流还原与分析物的量成正比。在优化的实验条件下，所提出的传感策略提供了从 10 到 10(4)ng mL(-1)的线性动态范围和 3ng mL(-1)的检测限，表明分析性能得到了提高。这可能使其成为蛋白质和其他分子的生物分析中的一种潜在替代方法。
