College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, 138 Dong-Ting Road, Changde 415000, Hunan, China.
Anal Chim Acta. 2010 Jul 26;674(1):27-31. doi: 10.1016/j.aca.2010.06.007. Epub 2010 Jun 15.
On the basis of copper-enhanced gold nanoparticle tags as an amplification approach, we introduced, in this paper, magnetic nanoparticles for further improving performance of electrochemical immunoassay by anodic stripping voltammetry (ASV) at a glassy-carbon electrode. Due to the use of antibody-immobilized magnetic nanoparticles, the immunoreaction between antibody and antigen takes place in a homogeneous bulk solution phase. Compared with traditional solid interface reaction, the proposed strategy can provide some advantages such as easy of separation, shorter analytical time, wider linear range, and lower detection limit. It was also successfully applied to HBsAg determination in a linear range of 0.1-1500 ng mL(-1) with a detection limit of 87 pg mL(-1). The proposed analytical strategy holds good selectivity, sensitivity and repeatability and also great promise for the extended application in the fields of clinical diagnosis, bio-affinity assay and environmental monitoring.
基于铜增强金纳米粒子标记作为一种放大方法，本文引入磁性纳米粒子，通过玻碳电极上的阳极溶出伏安法（ASV）进一步提高电化学免疫分析的性能。由于使用了抗体固定化磁性纳米粒子，抗体和抗原之间的免疫反应发生在均相的本体溶液相中。与传统的固-液界面反应相比，该策略具有易于分离、分析时间更短、线性范围更广、检测限更低等优点。该方法还成功地应用于 HBsAg 的测定，线性范围为 0.1-1500ngmL(-1)，检测限为 87pgmL(-1)。所提出的分析策略具有良好的选择性、灵敏度和重现性，在临床诊断、生物亲和性分析和环境监测等领域也具有广阔的应用前景。
