Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
Talanta. 2010 Nov 15;83(1):286-90. doi: 10.1016/j.talanta.2010.08.031. Epub 2010 Aug 27.
Rifamycin-capped (3-(2-O-β-cyclodextrin)-2-hydroxypropoxy)-propylsilyl-appended silica particles (RCD-HPS), a new type of substituted β-cyclodextrin-bonded chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC), have been synthesized by the treatment of bromoacetate-substituted-(3-(2-O-β-cyclodextrin)-2-hydroxypropoxy)-propylsilyl-appended silica particles (BACD-HPS) with rifamycin SV in anhydrous acetonitrile. The stationary phase is characterized by means of elemental analysis and Fourier-transform infrared spectroscopy. This new CSP has a chiral selector with two recognition sites: rifamycin and β-cyclodextrin (β-CD). The chromatographic behavior of RCD-HPS was studied with several disubstituted benzenes and some chiral drug compounds under reversed-phase HPLC mobile phase conditions. The results show that RCD-HPS has excellent selectivity for the separation of aromatic positional isomers and enantiomers of chiral compounds due to the cooperative functioning of rifamycin and β-CD.
利福霉素封端的（3-（2-O-β-环糊精）-2-羟丙氧基）丙基硅烷键合硅胶颗粒（RCD-HPS），一种新型取代β-环糊精键合手性固定相（CSP），用于高效液相色谱（HPLC），通过溴乙酸酯取代的（3-（2-O-β-环糊精）-2-羟丙氧基）丙基硅烷键合硅胶颗粒（BACD-HPS）与利福霉素 SV 在无水乙腈中的反应合成。固定相通过元素分析和傅里叶变换红外光谱进行表征。这种新型 CSP 具有两个识别位点的手性选择剂：利福霉素和β-环糊精（β-CD）。在反相 HPLC 流动相条件下，用几种二取代苯和一些手性药物化合物研究了 RCD-HPS 的色谱行为。结果表明，由于利福霉素和β-CD 的协同作用，RCD-HPS 对芳香族位置异构体和手性化合物对映体的分离具有优异的选择性。
