Voigt Niels, Makary Samy, Nattel Stanley, Dobrev Dobromir
Division of Experimental Cardiology, Department of Internal Medicine I--Cardiology, Angiology, Pneumology, Intensive Care and Hemostaseology, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer, Mannheim, Germany.
Methods Enzymol. 2010;484:653-75. doi: 10.1016/B978-0-12-381298-8.00032-0.
Vagal nerve stimulation can promote atrial fibrillation (AF) that requires activation of the acetylcholine (ACh)-gated potassium current I(K,ACh). In chronic AF (cAF), I(K,ACh) shows strong activity despite the absence of ACh or analogous pharmacological stimulation. This receptor-independent, constitutive I(K,ACh) activity is suggested to represent an atrial-selective anti-AF therapeutic target, but the underlying molecular mechanisms are unknown. This chapter provides an overview of the voltage-clamp techniques that can be used to study constitutive I(K,ACh) activity in atrial myocytes and summarizes briefly the current knowledge about the potential underlying mechanism(s) of constitutive I(K,ACh) activity in diseased heart.
迷走神经刺激可促进心房颤动（AF），这需要激活乙酰胆碱（ACh）门控钾电流I(K,ACh)。在慢性房颤（cAF）中，尽管没有ACh或类似的药理学刺激，I(K,ACh)仍表现出强烈的活性。这种不依赖受体的组成性I(K,ACh)活性被认为是一种心房选择性抗房颤治疗靶点，但其潜在的分子机制尚不清楚。本章概述了可用于研究心房肌细胞中组成性I(K,ACh)活性的电压钳技术，并简要总结了目前关于患病心脏中组成性I(K,ACh)活性潜在机制的认识。
