Department of Genetics, University of Pretoria, Pretoria, South Africa.
Fungal Biol. 2010 Nov-Dec;114(11-12):966-79. doi: 10.1016/j.funbio.2010.09.007. Epub 2010 Oct 8.
Chrysoporthe cubensis is one of the most important pathogens of Eucalyptus. Based on phylogenetic evidence and geographic origin, isolates of this fungus are known to reside in distinct 'South America' and 'Southeast Asia' clades. In this study, reproductive isolation amongst these isolates of C. cubensis was tested using gene flow statistics for 12 polymorphic loci, and to support these data, phylogenetic affiliations based on gene trees and a multigene phylogeny were used. Gene flow statistics between populations, and relative to the closely related Chrysoporthe austroafricana, were low and not significantly different (P<0.05). Additionally, phylogenetic analyses of DNA sequence data for four gene regions convincingly distinguished the two subclades of C. cubensis. Isolates in the Southeast Asian subclade are described in the new species, Chrysoporthe deuterocubensis. Chrysoporthe cubensis and C. deuterocubensis represent closely related fungi that are thought to be native to South America and Southeast Asia, respectively. A technique is presented that allows for rapid differentiation between these species and that will aid in quarantine procedures to limit their spread to new environments.
古巴栓孔菌是桉树的最重要病原菌之一。基于系统发育证据和地理起源，该真菌的分离株被认为存在于明显的“南美洲”和“东南亚”分支中。在这项研究中，使用 12 个多态性基因座的基因流统计数据来测试这些古巴栓孔菌分离株之间的生殖隔离，并使用基于基因树和多基因系统发育的系统发育关系来支持这些数据。种群之间的基因流统计数据以及与亲缘关系密切的 Chrysoporthe austroafricana 的相对基因流统计数据均较低，且无显著差异（P<0.05）。此外，对四个基因区域的 DNA 序列数据的系统发育分析令人信服地区分了古巴栓孔菌的两个亚分支。东南亚亚分支中的分离株被描述为新种，即 Chrysoporthe deuterocubensis。古巴栓孔菌和 Chrysoporthe deuterocubensis 代表密切相关的真菌，分别被认为是南美的和东南亚的本地种。本文提出了一种可快速区分这两个种的技术，这将有助于检疫程序限制它们向新环境的传播。
