Department of Marine Living Resources, Iranian National Institute for Oceanography, Tehran, Iran.
J Chromatogr A. 2010 Dec 3;1217(49):7642-7. doi: 10.1016/j.chroma.2010.10.031. Epub 2010 Oct 13.
A novel nano-structured copolymer of aniline and m-amino benzoic acid (m-ABA) was introduced as a coating of solid phase microextraction (SPME) of saturated-fatty acids in zooplanktons by coupling to gas chromatography-mass spectrometry (GC-MS). The nano-structured coating was prepared using co-polymerization of m-aminobenzoic acid and aniline. Improved temperature resistance (up to 350°C), relatively improved life time (more than 50 times) and satisfactory extraction efficiency were obtained by insertion of carboxylate groups into the framework of polyaniline. Different extraction parameters such as extraction temperature, extraction time, ionic strength, stirring rate and headspace volume were investigated and optimized. Single fiber repeatability and fiber-to-fiber reproducibility were <5.7% and <10.2%, respectively and the limits of detection varied from 0.01 (C14:0) to 6.07 μg L(-1) (C20:0). Correlation coefficients (R(2)) of the calibration curves ranged from 0.992 (C20:0) to 0.998 (C18:0) with a linearity from 0.5 to 200 μg mL(-1). The recoveries obtained ranged from 83% (C16:0) to 115% (C14:0).
一种新型的苯胺和间氨基苯甲酸（m-ABA）的纳米结构共聚物，通过与气相色谱-质谱联用（GC-MS）偶联，被引入作为浮游动物中饱和脂肪酸固相微萃取（SPME）的涂层。纳米结构涂层是通过间氨基苯甲酸和苯胺的共聚制备的。通过将羧酸盐基团插入聚苯胺的骨架中，得到了改进的耐高温性（高达 350°C）、相对延长的寿命（超过 50 倍）和令人满意的萃取效率。研究并优化了不同的萃取参数，如萃取温度、萃取时间、离子强度、搅拌速度和顶空体积。单纤维重复性和纤维间重现性分别<5.7%和<10.2%，检测限范围从 0.01（C14:0）到 6.07μg L(-1)（C20:0）。校准曲线的相关系数（R(2)）范围从 0.992（C20:0）到 0.998（C18:0），线性范围从 0.5 到 200μg mL(-1)。回收率范围从 83%（C16:0）到 115%（C14:0）。
