Preparation and evaluation of solid-phase microextraction fiber based on nano-structured copolymer of aniline and m-amino benzoic acid coating for the analysis of fatty acids in zooplanktons.

A novel nano-structured copolymer of aniline and m-amino benzoic acid (m-ABA) was introduced as a coating of solid phase microextraction (SPME) of saturated-fatty acids in zooplanktons by coupling to gas chromatography-mass spectrometry (GC-MS). The nano-structured coating was prepared using co-polymerization of m-aminobenzoic acid and aniline. Improved temperature resistance (up to 350°C), relatively improved life time (more than 50 times) and satisfactory extraction efficiency were obtained by insertion of carboxylate groups into the framework of polyaniline. Different extraction parameters such as extraction temperature, extraction time, ionic strength, stirring rate and headspace volume were investigated and optimized. Single fiber repeatability and fiber-to-fiber reproducibility were <5.7% and <10.2%, respectively and the limits of detection varied from 0.01 (C14:0) to 6.07 μg L(-1) (C20:0). Correlation coefficients (R(2)) of the calibration curves ranged from 0.992 (C20:0) to 0.998 (C18:0) with a linearity from 0.5 to 200 μg mL(-1). The recoveries obtained ranged from 83% (C16:0) to 115% (C14:0).