Horáková L, Lukovic L, Uraz V, Stolc S
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Physiol Bohemoslov. 1990;39(6):513-7.
The time course of lipid peroxidation was studied in the rat brain cortex after ischaemia and reperfusion. The ischaemia was induced by 4-hour occlusion of both common carotid arteries and was followed by reperfusion of different duration (10, 30 or 60 min). The extent of lipid peroxidation was determined by measurement of conjugated dienes (CD) and TBA reactive products. Maximal values of CD and TBA reactive products were found after 10- and 30-minute reperfusion. This indicated the most suitable time interval for studying the effect of antioxidants and oxygen radical scavengers in this model of brain ischaemia.
研究了大鼠脑皮质缺血再灌注后脂质过氧化的时间进程。通过双侧颈总动脉闭塞4小时诱导缺血，随后进行不同时长（10、30或60分钟）的再灌注。通过测量共轭二烯（CD）和硫代巴比妥酸反应产物来确定脂质过氧化的程度。在再灌注10分钟和30分钟后发现CD和硫代巴比妥酸反应产物的最大值。这表明在该脑缺血模型中研究抗氧化剂和氧自由基清除剂作用的最合适时间间隔。
