Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
Anticancer Res. 2010 Oct;30(10):4001-5.
Highly sensitive Coomassie brilliant blue SeePico™ Stain was applied for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). After staining with Flamingo™ Fluorescent Gel Stain, the images of the protein spots were analyzed, and 424 protein spots were detected. After washing with Milli-Q water three times, the gels were re-stained with SeePico™ Stain and the images of the protein spots were analyzed; 272 spots were detected. To assess whether SeePico™ Stain alters MS analysis, a spot was picked up and was analyzed by LC-MS/MS. The MS analysis showed good protein identification. These results show a possible role for SeePico™ Stain in cancer proteomics using 2-DE and MS.
高灵敏度考马斯亮蓝 SeePico™ 染色法用于二维凝胶电泳（2-DE）和液相色谱-串联质谱（LC-MS/MS）的蛋白质组学分析。使用 Flamingo™ 荧光凝胶染色法染色后，对蛋白质斑点的图像进行分析，检测到 424 个蛋白质斑点。用 Milli-Q 水洗涤三次后，用 SeePico™ 染色液重新染色并对蛋白质斑点的图像进行分析，检测到 272 个斑点。为了评估 SeePico™ 染色液是否改变 MS 分析，选择一个斑点进行 LC-MS/MS 分析。MS 分析显示良好的蛋白质鉴定。这些结果表明，SeePico™ 染色液在使用 2-DE 和 MS 的癌症蛋白质组学中可能具有作用。
