子宫内膜癌中 BAG-1 基因、ER 和 PR 的同形表达。
Isomorph expression of BAG-1 gene, ER and PR in endometrial cancer.
机构信息
Department of Virology, Papanikolaou Research Center of Oncology and Experimental Surgery, St. Savvas Regional Anticancer Oncology Hospital of Athens, Athens, Greece.
出版信息
Anticancer Res. 2010 Oct;30(10):4103-8.
BACKGROUND
BAG-1 isomorphs are regulating proteins with antiapoptotic action in endometrium. ERa and PRA isomorphs seem to have an important role in endometrial cancer.
PATIENTS AND METHODS
We investigated the expression of BAG-1, ERa and PRA isomorphs in endometrioid adenocarcinoma and we correlated them with clinicopathological findings of the tumor. Fresh endometrial tissues were obtained from 33 patients with endometrial carcinoma and 191 paraffin-embedded tissues were analyzed by real-time PCR and immunochemistry for BAG-1, ER and PR.
RESULTS
BAG-1 protein is expressed in both nucleus and cytoplasm. Grade 3 tumors were considered to have the highest intensity. Only 4 out of 79 samples showed intense expression of ERa, while 37 samples out of 72 samples strongly expressed PRA.
CONCLUSION
BAG-1 nuclear isomorph appeared more frequently in grade 2 tumors than in grade 1 and 3 tumors, and the cytoplasmatic isomorph was expressed more strongly than the nuclear one.
背景
BAG-1 同型物是具有抗凋亡作用的调节蛋白，存在于子宫内膜中。ERa 和 PRA 同型物似乎在子宫内膜癌中起重要作用。
患者与方法
我们研究了内胚层样腺癌中 BAG-1、ERa 和 PRA 同型物的表达，并将其与肿瘤的临床病理发现相关联。我们通过实时 PCR 和免疫化学法分析了 33 例子宫内膜癌患者的新鲜子宫内膜组织和 191 例石蜡包埋组织中 BAG-1、ER 和 PR 的表达。
结果
BAG-1 蛋白在细胞核和细胞质中均有表达。3 级肿瘤被认为具有最强的表达强度。在 79 个样本中仅有 4 个显示出 ERa 的强烈表达，而在 72 个样本中有 37 个强烈表达 PRA。
结论
BAG-1 核同型物在 2 级肿瘤中比在 1 级和 3 级肿瘤中更常见，细胞质同型物的表达强于核同型物。
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