UKNEQAS Parasitology, Department of Clinical Parasitology, Hospital for Tropical Diseases, London, UK.
J Clin Pathol. 2010 Dec;63(12):1112-5. doi: 10.1136/jcp.2010.077214. Epub 2010 Oct 29.
To examine performance in the UK National External Quality Assessment Scheme (UKNEQAS) for toxoplasma serology for evidence of discrepant results as compared with the predistribution and postdistribution results supplied by the toxoplasma reference laboratories.
Analysis of performance in the toxoplasma IgG and IgM schemes was made for the period 1994-2008 to look for trends in performance.
For the IgG scheme, a mean of 98% of participants obtained the correct result for detection of toxoplasma-specific antibody. The most common problem was failure to detect low levels of antibody. In some cases this was the result of participants deviating from the manufacturer's instructions and using higher cut-off levels. For the IgM scheme, an average of 95% of participants obtained the correct result for toxoplasma antibody detection. The most common problem was the failure of some enzyme immunoassay kits to detect specific toxoplasma IgM antibody, which was detected by the more sensitive immunosorbent agglutination assay.
Performance standards in the UKNEQAS toxoplasma serology schemes were high. The problems encountered have highlighted the importance of detecting low levels of antibody, adhering to the kit manufacturer's instructions and selecting an appropriate assay for the clinical situation.
通过与弓形虫参考实验室提供的分发前和分发后结果进行比较，考察英国国家外部质量评估计划（UKNEQAS）中弓形虫血清学检测的性能，以评估是否存在不一致的结果。
对 1994 年至 2008 年期间的弓形虫 IgG 和 IgM 方案的性能进行分析，以寻找性能变化趋势。
对于 IgG 方案，平均 98%的参与者获得了正确的检测结果，以检测弓形虫特异性抗体。最常见的问题是未能检测到低水平的抗体。在某些情况下，这是由于参与者偏离了制造商的说明并使用了更高的截止值。对于 IgM 方案，平均 95%的参与者获得了正确的检测结果，以检测弓形虫抗体。最常见的问题是一些酶免疫试剂盒未能检测到特定的弓形虫 IgM 抗体，而更敏感的免疫吸附凝集检测法则能检测到该抗体。
英国 NEQAS 弓形虫血清学方案的性能标准很高。所遇到的问题强调了检测低水平抗体、遵守试剂盒制造商的说明以及为临床情况选择适当的检测方法的重要性。
