Mycology and Botany Program, American Type Culture Collection (ATCC), 10801 University Blvd, Manassas, VA 20110, USA.
Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 30014, Taiwan.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2538-2542. doi: 10.1099/ijs.0.028860-0. Epub 2010 Oct 29.
Four arthroconidium-producing yeasts were isolated from the gut of wood-inhabiting tenebrionid and passalid beetles. The rRNA genes of these yeast strains were sequenced, compared and analysed. The sequence results and other taxonomic characterizations placed two of the strains into Trichosporon porosum, and the remaining strains, EH024(T) and EH026 which were isolated from Xylopinus saperdioides (Coleoptera: Tenebrionidae), into a novel species of the genus Trichosporon in the Porosum clade. Strain EN6S23 was independently isolated from forest soil in Taiwan and was identified as the same novel species based on identical sequences in the internal transcribed spacers (ITS) and the D1/D2 region of the LSU rRNA gene and similar physiological characteristics to those of strains EH024(T) and EH026. The three strains can assimilate cellulose and xylan as sole carbon source, and are clearly distinguished from their closest taxon, T. porosum, by 14 nt differences in the ITS and D1/D2 region. These strains did not reproduce sexually under the laboratory conditions tested. The novel species is proposed as Trichosporon xylopini sp. nov. (type strain EH024(T) = ATCC MYA-4670(T) = CBS 11841(T)).
从栖息在木材中的鞘翅目和象甲目的甲虫肠道中分离出了 4 株产节孢子的酵母。对这些酵母菌株的 rRNA 基因进行了测序、比较和分析。序列结果和其他分类特征将其中 2 株菌株归入 Trichosporon porosum，而其余 2 株菌株 EH024(T) 和 EH026 则从 Xylopinus saperdioides（鞘翅目：象甲科）中分离出来，归入 Porosum 分支的 Trichosporon 属的一个新种。菌株 EN6S23 是从台湾森林土壤中独立分离出来的，根据 ITS 内部转录间隔区和 LSU rRNA 基因的 D1/D2 区的相同序列以及与菌株 EH024(T) 和 EH026 相似的生理特征，被鉴定为相同的新种。这 3 株菌株可以将纤维素和木聚糖作为唯一的碳源同化，并且与它们最接近的分类群 T. porosum 有 14 个核苷酸的差异，在 ITS 和 D1/D2 区明显不同。这些菌株在实验室条件下没有进行有性繁殖。该新种被提议为 Trichosporon xylopini sp. nov.（模式菌株 EH024(T) = ATCC MYA-4670(T) = CBS 11841(T)）。
