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鲍氏假单胞菌,从土壤中分离得到。

Pseudomonas bauzanensis sp. nov., isolated from soil.

机构信息

Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.

China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

出版信息

Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2333-2337. doi: 10.1099/ijs.0.026104-0. Epub 2010 Oct 29.

Abstract

A Gram-negative, aerobic, motile rod, designated BZ93(T), was isolated from soil from an industrial site. The strain grew at 5-30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ93(T) was related to members of the genus Pseudomonas and was related most closely to Pseudomonas xiamenensis C10-2(T) (97.8 % 16S rRNA gene sequence similarity) and Pseudomonas pertucinogena IFO 14163(T) (97.4 %). The predominant cellular fatty acids of strain BZ93(T) were C(18 : 1)ω7c (54.8 %), summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 10.3 %), C(16 : 0) (9.9 %) and C(17 : 0) cyclo (7.4 %). The major quinone was ubiquinone 9. The major phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. The genomic DNA G+C content was 61.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Pseudomonas bauzanensis sp. nov., is proposed. The type strain is BZ93(T) ( = DSM 22558(T)  = CGMCC 1.9095(T) = LMG 26048(T)).

摘要

一株革兰氏阴性、需氧、能动的杆菌,被命名为 BZ93(T),从工业场地的土壤中分离得到。该菌株在 5-30°C 下生长。基于 16S rRNA 基因序列的系统发育分析表明,菌株 BZ93(T)与假单胞菌属的成员有关,与厦门假单胞菌 C10-2(T)(97.8% 16S rRNA 基因序列相似性)和 pertucinogena IFO 14163(T)(97.4%)最为密切。菌株 BZ93(T)的主要细胞脂肪酸为 C(18:1)ω7c(54.8%)、特征性脂肪酸 3(C(16:1)ω7c 和/或 iso-C(15:0) 2-OH;10.3%)、C(16:0)(9.9%)和 C(17:0) 环(7.4%)。主要醌为泛醌 9。主要磷脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油和一种未知的磷脂。基因组 DNA G+C 含量为 61.8 mol%。基于表型特征、系统发育分析和 DNA-DNA 同源性,提出了一个新的物种,即 Bauzanensis 假单胞菌。模式菌株为 BZ93(T)(DSM 22558(T)  = CGMCC 1.9095(T)  = LMG 26048(T))。

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