Wang Yong-Xia, Xiao Wei, Dong Ming-Hua, Zhao Qin, Li Zhi-Ying, Lai Yong-Hong, Cui Xiao-Long
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People's Republic of China.
Antonie Van Leeuwenhoek. 2014 Aug;106(2):253-60. doi: 10.1007/s10482-014-0189-1. Epub 2014 May 23.
Two Gram-stain negative, moderately halophilic, aerobic, motile bacteria, designated strains YIM QH88(T) and YIM QH103, were isolated from the Qiaohou salt mine in Yunnan, southwest China. Cells of the strains were observed to be rod-shaped and produce creamy-coloured colonies. Growth of the two strains was observed at 10-45 °C (optimum 25-37 °C), at pH 6.0-10.0 (optimum 7.0-8.0), and in the presence of 0.5-20 % (w/v) NaCl (optimum 2-6 %). The two strains were found to contain summed feature 8 (C18:1 ω7c/ω6c), C19:0 cyclo ω8c and C16:0 as the major cellular fatty acids. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipid. The G+C content of the genomic DNA of strains YIM QH88(T) and YIM QH103 were determined to be 64.6 and 64.2 mol%, respectively, and the predominant respiratory quinone detected was ubiquinone 9. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM QH88(T) and YIM QH103 formed a distinct lineage within the genus Halomonas and were most closely related to Halomonas pantelleriensis DSM 9661(T) with 97.3 and 97.5 % of 16S rRNA sequence similarity respectively. The DNA-DNA hybridization relatedness value for strains YIM QH88(T) and YIM QH103 was 95.2 ± 0.8 %. The levels of DNA-DNA relatedness between each of these two strains and the type strains of phylogenetically closely related Halomonas species were clearly below 70 %. On the basis of their phylogenetic analysis, DNA-DNA hybridization relatedness, phenotypic and chemotaxonomic characteristics, strains YIM QH88(T) and YIM QH103 should be classified as a novel species of the genus Halomonas, for which the name Halomonas qiaohouensis sp. nov. is proposed. The type strain is YIM QH88(T) (=DSM 26770(T) =CCTCC AB 2012965(T)).
从中国西南部云南省乔后盐矿中分离出两株革兰氏染色阴性、中度嗜盐、需氧、具运动性的细菌，分别命名为菌株YIM QH88(T)和YIM QH103。观察到菌株细胞呈杆状，形成奶油色菌落。两株菌在10-45 °C（最适温度25-37 °C）、pH 6.0-10.0（最适pH 7.0-8.0）以及0.5-20%（w/v）NaCl（最适浓度2-6%）条件下均能生长。发现这两株菌的主要细胞脂肪酸为特征性总和8（C18:1 ω7c/ω6c）、C19:0环ω8c和C16:0。极性脂类鉴定为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和未知磷脂。菌株YIM QH88(T)和YIM QH103基因组DNA的G+C含量分别测定为64.6和64.2 mol%，检测到的主要呼吸醌为泛醌9。基于16S rRNA基因序列的系统发育分析表明，菌株YIM QH88(T)和YIM QH103在嗜盐单胞菌属内形成一个独特的分支，与潘泰莱里亚嗜盐单胞菌DSM 9661(T)关系最为密切，16S rRNA序列相似性分别为97.3%和97.5%。菌株YIM QH88(T)和YIM QH103的DNA-DNA杂交相关性值为95.2 ± 0.8%。这两株菌与系统发育关系密切的嗜盐单胞菌属模式菌株之间的DNA-DNA相关性水平明显低于70%。基于系统发育分析、DNA-DNA杂交相关性、表型和化学分类特征，菌株YIM QH88(T)和YIM QH103应被分类为嗜盐单胞菌属的一个新物种，为此提出新名称乔后嗜盐单胞菌（Halomonas qiaohouensis sp. nov.）。模式菌株为YIM QH88(T)（=DSM 26770(T)=CCTCC AB 2012965(T)）。
