Institute of Organic Chemistry and Biochemistry, Prague 6, 166 10, Czech Republic.
Chirality. 2010;22 Suppl 1:E47-55. doi: 10.1002/chir.20851.
The compounds I-IV derived from α-D-cyclodextrin moiety by bridging and/or interconnecting with various patterns of disulfide bonds were chosen as models for the spectroscopic study of conformation of the disulfide bridge. The energy gap between the disulfide and cyclodextrin's electronic transitions allows us to investigate absorption and electronic circular dichroism spectra without disturbing spectral overlaps with amides or aromatic amino acids in peptides or proteins. Raman optical activity (ROA) spectra were measured and the bands due to S-S and C-S stretching motion identified. Comparison with the quantum mechanical calculations of simple models indicates that sense of disulfide twist follows sign of the measured S-S ROA band.
我们选择了由α-D-环糊精部分通过桥连和/或各种二硫键模式相互连接衍生而来的化合物 I-IV，作为二硫键构象光谱研究的模型。二硫键和环糊精之间的能隙允许我们在不干扰肽或蛋白质中的酰胺或芳香族氨基酸光谱重叠的情况下，研究吸收和电子圆二色性光谱。测量了拉曼光学活性（ROA）光谱，并确定了 S-S 和 C-S 伸缩运动的谱带。与简单模型的量子力学计算进行比较表明，二硫键扭转的方向与所测量的 S-S ROA 带的符号一致。
