Xu Yan-Ling, Zhao Juan, Ma Rui-Yu, Xu Shu-Mei
Department of Physiology, Tianjin Medical University, Tianjin 300070, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Aug;26(3):302-6.
To observe the influence of H102 on the expression of amyloid protein and amyloid precursor protein in the hippocampus of APP695 transgenic mice.
The 9-month-old APP695 transgenic mice were randomly divided into the model group and the H102 group; C57BL/6J mice were adopted as normal control group. The H102 group were injected with H102 in a dose of 3 microl/per mouse in lateral ventricle, once a day, for ten days; while the model group and the control group were injected with saline. The hippocampus and temporal cortex of the brain sections from transgenic mice and wild type female mice were subjected to immunohistochemistry and Congo red histological staining, and observed the difference of the protein expression under microscope. The expression of the APP protein was detected by Western blot.
Abeta and APP immunohistochemistry showed density of positive cell in the CA1 region of hippocampus of control group were less than model group. H102 peptide reduced the area, and density of positive cells. Congo red staining showed there were lots of amyloid plagues in the brains of model mice but not in the brains of normal control. And the Western blot showed the content of the APP protein of the model group was much higher than the H102 group. H102 significantly decreased the amyloid plagues.
The expression of APP, Abeta are increased in APP695 transgenic mice, and H102 can decrease the level of APP, Abeta in transgenic mice.
观察H102对APP695转基因小鼠海马中淀粉样蛋白及淀粉样前体蛋白表达的影响。
将9月龄APP695转基因小鼠随机分为模型组和H102组；采用C57BL/6J小鼠作为正常对照组。H102组小鼠侧脑室注射H102，剂量为每只小鼠3微升，每日1次，共10天；模型组和对照组注射生理盐水。对转基因小鼠和野生型雌性小鼠脑切片的海马和颞叶皮质进行免疫组织化学和刚果红组织学染色，显微镜下观察蛋白表达差异。采用蛋白质印迹法检测APP蛋白的表达。
β-淀粉样蛋白（Aβ）和APP免疫组织化学显示，对照组海马CA1区阳性细胞密度低于模型组。H102肽降低了阳性细胞的面积和密度。刚果红染色显示，模型小鼠脑内有大量淀粉样斑块，而正常对照组脑内无。蛋白质印迹法显示，模型组APP蛋白含量明显高于H102组。H102显著减少了淀粉样斑块。
APP695转基因小鼠中APP、Aβ表达增加，H102可降低转基因小鼠中APP、Aβ水平。
