Boudreaux Mary K, Osborne Christina D, Herre Adam C, Rivera Elliot Ramos, Spangler Elizabeth A
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
Vet Clin Pathol. 2010 Dec;39(4):417-23. doi: 10.1111/j.1939-165X.2010.00256.x. Epub 2010 Oct 7.
Platelet size is relatively uniform in mammals except for domestic cats. Uniform platelet production by megakaryocytes can be disrupted if microtubule assembly or dynamics is impaired. Mutations in the gene encoding β1-tubulin have been documented in dogs and people, and the resulting microtubule effects have been associated with production of large platelets.
The objectives of this study were to evaluate morphology of platelets on feline blood smears, determine the gene sequences encoding β1-tubulin in members of the family Felidae, and compare the findings with those in other mammalian species to determine whether predicted structural differences in β1-tubulin that might affect microtubule stability or assembly were present.
At least 100 platelets/smear on blood smears from 15 domestic cats and 88 big cats were evaluated to assess platelet size variability. Platelet-derived cDNA obtained from a domestic cat and genomic DNA isolated from blood samples of domestic cats and other members of the family Felidae were analyzed by PCR using primers specific for β1-tubulin. Gene sequences obtained were compared with those of other common mammals.
Two differences in gene sequence were found in a highly conserved region encoding the M loop of β1-tubulin in members of the family Felidae compared with sequences from other species. Platelet size variation was present in big cats and domestic cats. In addition, a rare amino acid change was documented in the C-terminal region encoding the H11 helix in domestic cats.
Members of the family Felidae have an altered M loop region in β1-tubulin compared with other mammals. This variation may contribute to the observed platelet size variability.
除家猫外，哺乳动物的血小板大小相对均匀。如果微管组装或动力学受损，巨核细胞产生均匀血小板的过程可能会受到干扰。在狗和人类中已记录到编码β1-微管蛋白的基因突变，由此产生的微管效应与大血小板的产生有关。
本研究的目的是评估猫科动物血液涂片上血小板的形态，确定猫科动物成员中编码β1-微管蛋白的基因序列，并将结果与其他哺乳动物物种进行比较，以确定β1-微管蛋白中可能影响微管稳定性或组装的预测结构差异是否存在。
对15只家猫和88只大型猫科动物的血液涂片上至少100个血小板/涂片进行评估，以评估血小板大小的变异性。使用针对β1-微管蛋白的特异性引物，通过PCR分析从一只家猫获得的血小板衍生cDNA以及从家猫和猫科动物其他成员的血液样本中分离的基因组DNA。将获得的基因序列与其他常见哺乳动物的序列进行比较。
与其他物种的序列相比，在猫科动物成员中编码β1-微管蛋白M环的高度保守区域发现了两个基因序列差异。大型猫科动物和家猫均存在血小板大小变异。此外，在家猫中编码H11螺旋的C末端区域记录到一个罕见的氨基酸变化。
与其他哺乳动物相比，猫科动物成员的β1-微管蛋白M环区域发生了改变。这种变异可能导致观察到的血小板大小变异性。
