染色质结合 PCNA 作为急性淋巴细胞白血病或多发性骨髓瘤患者单核细胞中的 S 期标志物。
Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma.
机构信息
Department of Medical Radiobiology, Faculty of Medicine, University Duisburg-Essen, Essen, Germany.
出版信息
Cell Prolif. 2010 Dec;43(6):579-83. doi: 10.1111/j.1365-2184.2010.00707.x.
OBJECTIVES
Proliferating cell nuclear antigen (PCNA) has often been used as a marker to aid assessment of tumour growth fraction. This paper addresses the question of whether it can be used as an S-phase marker, when the non-chromatin-bound form of the protein is removed by pepsin treatment.
MATERIALS AND METHODS
Cytofluorometric measurements were carried out after immunofluorescence staining of PCNA and counterstaining of DNA. S-phase fraction was determined with the help of windows on PCNA versus DNA scattergrams, or mathematically from DNA histograms.
RESULTS
S-phase fractions obtained using the two methods correlated well, but did not always agree, exact discrepancies depending on the mathematical model used for histogram analysis.
CONCLUSIONS
Determination of S-phase fractions with the help of PCNA immunofluorescence staining is possible, and probably more reliable than calculation of S-fractions from DNA histograms. It thus offers an alternative to assays involving BrdU labelling in vivo.
目的
增殖细胞核抗原 (PCNA) 常被用作辅助评估肿瘤生长分数的标志物。本文探讨了在使用胃蛋白酶处理去除非染色质结合形式的蛋白质后，PCNA 是否可被用作 S 期标志物的问题。
材料与方法
通过 PCNA 免疫荧光染色和 DNA 复染后进行细胞荧光测量。使用 PCNA 与 DNA 散点图上的窗口或从 DNA 直方图中进行数学计算来确定 S 期分数。
结果
两种方法获得的 S 期分数相关性良好，但并不总是一致，具体差异取决于用于直方图分析的数学模型。
结论
通过 PCNA 免疫荧光染色确定 S 期分数是可行的，并且可能比从 DNA 直方图计算 S 分数更可靠。因此，它为涉及体内 BrdU 标记的测定提供了一种替代方法。
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