Departamento de Botânica, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, Brazil.
Plant Biol (Stuttg). 2010 Nov;12(6):863-70. doi: 10.1111/j.1438-8677.2009.00304.x.
Leaf glands of Diplopterys pubipetala were studied with light and electron microscopy. Aspects of their secretion, visitors and phenology were also recorded. Glands occur along the margin, at the apex and at the base of the leaf blade. All the glands begin secretion when the leaf is still very young, and secretion continues during leaf expansion. The highest proportion of young leaves coincides with the beginning of flowering. The glucose-rich secretion is collected by Camponotus ants, which patrol the newly formed vegetative and reproductive branches. All the glands are sessile, partially set into the mesophyll, and present uniseriate epidermis subtended by nonvascularised parenchyma. The glands at the apex and base are larger and also consist of vascularised subjacent parenchyma. The cytoplasm of epidermal and parenchyma cells has abundant mitochondria, polymorphic plastids filled with oil droplets and a few starch grains. Golgi bodies and endoplasmic reticulum are more abundant in the epidermal cells. The parenchyma cells of the subjacent region contain chloroplasts and large vacuoles. Plasmodesmata connect all the nectary cells. The zinc iodide-osmium tetroxide (ZIO) method revealed differences in the population of organelles between epidermal cells, as well as between epidermal cells and parenchyma cells. Ultrastructural results indicate that leaf glands of D. pubipetala can be classified as mixed secretory glands. However, the secretion released by these glands is basically hydrophilic and composed primarily of sugars, hence these glands function as nectaries.
对闭鞘姜科闭鞘姜属植物的叶腺进行了光镜和电镜研究。同时还记录了叶腺的分泌、访花者和物候学特征。叶腺沿叶片边缘、叶尖和叶基分布。叶片尚未完全展开时，所有叶腺就开始分泌，并且在叶片伸展过程中持续分泌。幼叶数量最多的时期与花期开始时间相吻合。富含葡萄糖的分泌物被Camponotus 蚂蚁收集，蚂蚁在新形成的营养和生殖枝上巡逻。所有叶腺均无柄，部分嵌入叶肉，具有单层表皮，下方为无维管束的薄壁组织。叶尖和叶基的叶腺较大，并且还包括下方有血管化的薄壁组织。表皮细胞和薄壁组织细胞的细胞质中含有丰富的线粒体、充满油滴的多形质体和少量淀粉粒。高尔基复合体和内质网在表皮细胞中更为丰富。下方区域的薄壁组织细胞含有叶绿体和大液泡。胞间连丝将所有蜜腺细胞连接在一起。锌碘酸-锇酸（ZIO）法揭示了表皮细胞以及表皮细胞和薄壁组织细胞之间细胞器群体的差异。超微结构结果表明，D. pubipetala 的叶腺可归类为混合分泌腺。然而，这些腺释放的分泌物基本上是亲水性的，主要由糖组成，因此这些腺起蜜腺的作用。
