Department of Biology, University of Western Ontario, London, ON, Canada.
Plant Biotechnol J. 2011 May;9(4):434-44. doi: 10.1111/j.1467-7652.2010.00563.x. Epub 2010 Oct 8.
Although many different crop species have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of four recombinant proteins (i.e. human erythropoietin and interleukin-10, an antibody against Pseudomonas aeruginosa, and a hyperthermostable α-amylase) was evaluated in numerous species and cultivars of Nicotiana. Whereas the transient level of recombinant protein accumulation varied significantly amongst the different Nicotiana plant hosts, the variety of Nicotiana had little practical impact on the recombinant protein concentration in stable transgenic plants. In addition, this study examined the growth rate, amount of leaf biomass, total soluble protein levels and the alkaloid content of the various Nicotiana varieties to establish the best plant platform for commercial production of recombinant proteins. Of the 52 Nicotiana varieties evaluated, Nicotiana tabacum (cv. I 64) produced the highest transient concentrations of recombinant proteins, in addition to producing a large amount of biomass and a relatively low quantity of alkaloids, probably making it the most effective plant host for recombinant protein production.
尽管许多不同的作物物种已被用于生产各种疫苗、抗体、生物制药和工业酶，但烟草在生产重组蛋白方面有着最成熟的历史。为了进一步提高烟草平台异源蛋白的产量，我们评估了四种重组蛋白（即人红细胞生成素和白细胞介素-10、抗铜绿假单胞菌抗体和嗜热α-淀粉酶）在多种烟草物种和品种中的瞬时和稳定表达。尽管不同的烟草植物宿主中重组蛋白积累的瞬时水平有很大差异，但烟草品种对稳定转化植物中重组蛋白的浓度几乎没有实际影响。此外，本研究还考察了各种烟草品种的生长速度、叶片生物量、总可溶性蛋白水平和生物碱含量，以确定用于商业生产重组蛋白的最佳植物平台。在评估的 52 个烟草品种中，烟草（cv. I 64）产生了最高的瞬时重组蛋白浓度，同时产生了大量的生物量和相对较低的生物碱含量，这使其成为用于生产重组蛋白最有效的植物宿主。
