鉴定默克尔细胞谱系的表皮祖细胞。
Identification of epidermal progenitors for the Merkel cell lineage.
机构信息
Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA.
出版信息
Development. 2010 Dec;137(23):3965-71. doi: 10.1242/dev.055970. Epub 2010 Nov 1.
Epithelial stem cells in adult mammalian skin are known to maintain epidermal, follicular and sebaceous lineages during homeostasis. Recently, Merkel cell mechanoreceptors were identified as a fourth lineage derived from the proliferative layer of murine skin epithelium; however, the location of the stem or progenitor population for Merkel cells remains unknown. Here, we have identified a previously undescribed population of epidermal progenitors that reside in the touch domes of hairy skin, termed touch dome progenitor cells (TDPCs). TDPCs are epithelial keratinocytes and are distinguished by their unique co-expression of α6 integrin, Sca1 and CD200 surface proteins. TDPCs exhibit bipotent progenitor behavior as they give rise to both squamous and neuroendocrine epidermal lineages, whereas the remainder of the α6(+) Sca1(+) CD200(-) epidermis does not give rise to Merkel cells. Finally, TDPCs possess a unique transcript profile that appears to be enforced by the juxtaposition of TDPCs with Merkel cells within the touch dome niche.
成年哺乳动物皮肤中的上皮干细胞在体内平衡过程中维持表皮、毛囊和皮脂腺谱系。最近，Merkel 细胞机械感受器被鉴定为源自鼠皮肤上皮增殖层的第四个谱系；然而，Merkel 细胞的干细胞或祖细胞群体的位置仍然未知。在这里，我们鉴定了一个以前未描述的位于多毛皮肤触盘的表皮祖细胞群体，称为触盘祖细胞（TDPC）。TDPC 是上皮角质形成细胞，其独特之处在于其共同表达α6 整联蛋白、Sca1 和 CD200 表面蛋白。TDPC 表现出双能祖细胞行为，因为它们产生鳞状和神经内分泌表皮谱系，而其余的α6(+)Sca1(+)CD200(-)表皮不会产生 Merkel 细胞。最后，TDPC 具有独特的转录谱，这似乎是由 TDPC 与 Merkel 细胞在触盘龛位中的毗邻所决定的。
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