培养南极线虫默里盘咽线虫。

Culturing the Antarctic nematode Plectus murrayi.

作者信息

Adhikari Bishwo N, Tomasel Cecilia M, Li Grace, Wall Diana H, Adams Byron J

机构信息

Department of Biology and Evolutionary Ecology Laboratories, Brigham Young University, Provo, UT 84602, USA.

出版信息

Cold Spring Harb Protoc. 2010 Nov 1;2010(11):pdb.prot5522. doi: 10.1101/pdb.prot5522.

DOI:10.1101/pdb.prot5522

PMID:21041395

Abstract

The Antarctic terrestrial nematode Plectus murrayi is an excellent model organism for the study of stress response mechanisms in various types of environmental conditions. In this procedure, we describe a method for culturing P. murrayi extracted from soil and sediment samples from the McMurdo (MCM) Dry Valleys of Antarctica. Nematodes are cultured on sand agar plates and incubated at various temperatures, and feed on bacteria growing on the agar media. They can be subcultured and stored at 15°C for >2 mo. This method is easy to carry out and can produce nematodes in quantities sufficient for ecological and molecular studies.

摘要

南极陆地线虫默里普列克线虫是研究各种环境条件下应激反应机制的优秀模式生物。在本实验步骤中，我们描述了一种培养从南极麦克默多（MCM）干谷土壤和沉积物样本中提取的默里普列克线虫的方法。线虫在砂琼脂平板上培养，在不同温度下孵育，并以琼脂培养基上生长的细菌为食。它们可以传代培养并在15°C下储存超过2个月。该方法易于实施，能够产生足够数量的线虫用于生态学和分子研究。