Dubey Manish, Jablin Michael S, Smith Hillary, Majewski Jarosław
Lujan Neutron Scattering Center, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Acta Crystallogr D Biol Crystallogr. 2010 Nov;66(Pt 11):1237-43. doi: 10.1107/S090744491001766X. Epub 2010 Oct 20.
The nonperturbative nature of neutron reflectometry (NR) coupled with its isotopic sensitivity has made it an ideal candidate for the study of model biological membranes at the solid-liquid interface. In this article, methods are presented for the creation and characterization of supported model membranes which can mimic many of the critical attributes of cell membranes. It is demonstrated that NR can characterize the structure, composition and organization of model membranes deposited on solid, nanoporous and polymer supports. Additionally, in situ NR measurements of the interactions between model membranes and external stimuli are presented. Finally, an investigation of the adherence region of live mouse fibroblast cells is described.
中子反射测量技术（NR）的非微扰性质及其同位素敏感性使其成为研究固液界面处模型生物膜的理想选择。本文介绍了用于创建和表征支撑型模型膜的方法，这些模型膜可以模拟细胞膜的许多关键特性。结果表明，NR能够表征沉积在固体、纳米多孔和聚合物支撑体上的模型膜的结构、组成和组织。此外，还展示了对模型膜与外部刺激之间相互作用的原位NR测量。最后，描述了对活的小鼠成纤维细胞黏附区域的研究。
