Research Center for Gastroenterology and Liver Diseases, Shaheed Beheshti Medical University, Tehran, Iran.
Digestion. 2011;83(1-2):65-75. doi: 10.1159/000320690. Epub 2010 Oct 29.
It has been frequently shown that p53 alterations have an important role in the development of gastric cancers but there is no data on p53 alteration in gastric cancer and its precancerous lesions from Iran although this country experiences one of the highest gastric cancer incidence and mortality rates in the world. The purpose of this study was to do a comprehensive assessment of p53 alterations in the Iranian population of gastritis patients and to evaluate the association between p53 alterations, microsatellite status and clinicopathological aspects.
After DNA extraction, PCR sequencing was done for exons 2-7. Also microsatellite status was evaluated using five microsatellite markers: NR-27, NR-21, NR-24, BAT-25 and BAT-26.
The highest rate of alteration was seen in codons 72 (85.6%, SNP) and 248 (30.9%, mutation). Also, we found 2 new mutations in codons 9 and 146. In contrast with previous work, transition at the CpG codons was relatively rare. Nucleotide alterations were more prevalent in the Helicobacter pylori-positive group but not significantly. Neither nuclear staining for p53 protein nor microsatellite instability was seen in gastritis lesions.
p53 alterations might contribute to the pathogenesis of gastritis and perhaps gastric cancer in Iran. However, the different spectrum seen here implies other mechanism(s) in gastritis and gastric cancer development in the Iranian population.
已经频繁地表明，p53 改变在胃癌的发展中起着重要作用，但伊朗尚无关于胃癌及其癌前病变中 p53 改变的数据，尽管该国的胃癌发病率和死亡率是世界上最高的之一。本研究的目的是全面评估伊朗胃炎患者人群中 p53 的改变，并评估 p53 改变、微卫星状态与临床病理方面之间的关系。
提取 DNA 后，对外显子 2-7 进行 PCR 测序。还使用五个微卫星标记物：NR-27、NR-21、NR-24、BAT-25 和 BAT-26 来评估微卫星状态。
改变率最高的是密码子 72（85.6%，SNP）和 248（30.9%，突变）。此外，我们在密码子 9 和 146 中发现了 2 个新突变。与之前的工作相比，CpG 密码子的转换相对较少。核苷酸改变在幽门螺杆菌阳性组中更为常见，但无统计学意义。胃炎病变中均未见 p53 蛋白核染色或微卫星不稳定性。
p53 改变可能有助于伊朗胃炎和胃癌的发病机制。然而，这里所见的不同谱表明，在伊朗人群中，胃炎和胃癌发展有其他机制。
