Fanslow W C, Clifford K, VandenBos T, Teel A, Armitage R J, Beckmann M P
Immunex Corporation, Seattle, Washington 98101.
Cytokine. 1990 Nov;2(6):398-401. doi: 10.1016/1043-4666(90)90047-w.
Murine biological fluids and murine cell culture supernatants were analyzed for the presence of soluble murine interleukin 4 receptor (sIL4R) with the use of two monoclonal antibodies directed against the receptor. Mouse urine, serum, ascitic fluid, and cell culture supernatants contained varying levels of immunoreactive protein. All of the immunoreactive protein possessed interleukin 4 (IL 4) binding activity. Following partial purification of ascitic fluid a protein was isolated that binds IL 4 with high affinity. This data is consistent with the fact that murine biological fluids contain a soluble version of the murine IL 4 receptor that arises via secretion of the soluble receptor and/or via shedding of the extracellular portion of the full-length receptor from the cell surface.
利用两种针对该受体的单克隆抗体，对小鼠生物体液和小鼠细胞培养上清液进行分析，以检测可溶性小鼠白细胞介素4受体（sIL4R）的存在。小鼠尿液、血清、腹水和细胞培养上清液中含有不同水平的免疫反应性蛋白。所有免疫反应性蛋白均具有白细胞介素4（IL 4）结合活性。对腹水进行部分纯化后，分离出一种与IL 4具有高亲和力的蛋白。该数据与以下事实一致，即小鼠生物体液中含有小鼠IL 4受体的可溶性形式，其通过可溶性受体的分泌和/或全长受体细胞外部分从细胞表面的脱落而产生。
