Misuno N I, Osipovich O A, Sudarikov A B, Idelson G L, Kolesnikova T S, Panyutich A V, Voitenok N N
Byelorussian Institute of Hematology and Blood Transfusion, Minsk, USSR.
Cytokine. 1990 Nov;2(6):464-9. doi: 10.1016/1043-4666(90)90056-y.
The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus.
本研究旨在评估循环人血单核细胞中肿瘤坏死因子（TNF）-α mRNA和蛋白的存在情况，并研究通过连续Percoll梯度分级分离法分离的人单核细胞中TNF-α基因的表达。采用直接从血样中分离RNA的技术来研究循环人血白细胞中TNF-α mRNA的表达。结果显示，健康供者的人血白细胞中既不存在预先合成的TNF-α mRNA池，也不存在TNF-α蛋白。研究发现，用环己酰亚胺进行早期预处理会干扰金黄色葡萄球菌诱导TNF-α mRNA的表达。
