Department of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, Bologna, Italy.
Int J Mol Med. 2010 Dec;26(6):913-8. doi: 10.3892/ijmm_00000542.
Regulator of calcineurin 3 (RCAN3) belongs to the human RCAN gene family, which also includes RCAN1 and RCAN2. All three members interact with and inhibit calcineurin. Based on this effect, several studies have demonstrated a role for RCAN1 and RCAN2 on inflammation, using human umbilical vein endothelial cells (HUVECs) as a model. RCAN1 and 2 are strongly induced by vascular endothelial growth factor (VEGF), inhibit cell proliferation and down-regulate many pro-inflammatory and pro-angiogenic genes. The present work is the first study to investigate the role of RCAN3 on inflammation in HUVECs. RCAN3 isoforms have been characterized and quantified in HUVECs; only those with the same frame are expressed and show a peculiar expression pattern. RCAN3 inhibits HUVEC proliferation both basally and under VEGF or phorbol 12-myristate 13-acetate-stimulated conditions, however it does not modulate gene expression of the chosen inflammatory genes. Results indicate an interesting role for RCAN3 in modulating HUVEC proliferation, independently from the inflammatory and angiogenic processes.
钙调神经磷酸酶调节蛋白 3（RCAN3）属于人类 RCAN 基因家族，该家族还包括 RCAN1 和 RCAN2。这三个成员相互作用并抑制钙调神经磷酸酶。基于这一作用，几项研究使用人脐静脉内皮细胞（HUVEC）作为模型，证明了 RCAN1 和 RCAN2 在炎症中的作用。RCAN1 和 2 强烈地被血管内皮生长因子（VEGF）诱导，抑制细胞增殖并下调许多促炎和促血管生成基因。本研究首次探讨了 RCAN3 在 HUVEC 炎症中的作用。RCAN3 异构体在 HUVEC 中得到了表征和定量；只有那些具有相同框架的异构体被表达，并表现出特殊的表达模式。RCAN3 抑制 HUVEC 的增殖，无论是在基础条件下还是在 VEGF 或佛波醇 12-肉豆蔻酸 13-乙酸刺激条件下，但它不调节所选炎症基因的表达。结果表明，RCAN3 在调节 HUVEC 增殖方面具有有趣的作用，这与炎症和血管生成过程无关。
