Jiangsu Key Laboratory of Molecular Medicine, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
Phytother Res. 2011 May;25(5):675-80. doi: 10.1002/ptr.3320. Epub 2010 Oct 29.
Cordyceps sinensis as a well-known traditional Chinese tonic has many therapeutic functions. In the present study, an acid polysaccharide (APS) was isolated from cultivated Cordyceps mycelia by ion-exchange and sizing chromatography. The protective capacity of APS against H(2)O(2)-induced oxidative damage in rat pheochromocytoma PC12 cells was investigated by measuring cell viability, lactate dehydrogenase (LDH) release, antioxidant enzyme activity, malondialdehyde (MDA) levels and intracellular accumulation of reactive oxygen species (ROS) and Ca(2+). The results demonstrated that pretreatment of PC12 cells with APS, prior to H(2)O(2) exposure, significantly increased the survival of cells and the activities of glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), and reduced the levels of LDH and MDA. Intracellular accumulation of reactive oxygen species (ROS) and Ca(2+) were also inhibited by APS treatment. In conclusion, APS was found to increase the cellular antioxidant defence capacity, thereby protecting PC12 cells against oxidative stress.
蛹虫草作为一种著名的中药补品，具有多种治疗功能。本研究采用离子交换和凝胶过滤色谱法从蛹虫草菌丝体中分离出一种酸性多糖（APS）。通过测定细胞活力、乳酸脱氢酶（LDH）释放、抗氧化酶活性、丙二醛（MDA）水平以及细胞内活性氧（ROS）和 Ca(2+)的积累，研究了 APS 对 H(2)O(2)诱导的大鼠嗜铬细胞瘤 PC12 细胞氧化损伤的保护作用。结果表明，APS 预处理 PC12 细胞，然后暴露于 H(2)O(2)，可显著提高细胞存活率和谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性，降低 LDH 和 MDA 的水平。APS 处理还抑制了细胞内活性氧（ROS）和 Ca(2+)的积累。综上所述，APS 可增强细胞抗氧化防御能力，从而保护 PC12 细胞免受氧化应激。
