人骨髓间充质干细胞冻干初步研究。
Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells.
机构信息
Refrigeration and Cryogenic Engineering Institute, Zhejiang University, Hangzhou, China.
出版信息
J Zhejiang Univ Sci B. 2010 Nov;11(11):889-94. doi: 10.1631/jzus.B1000184.
Abstract
Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33±13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.
摘要
长期保存和便于运输人类骨髓间充质干细胞（hBM-MSCs）将有助于它们在医疗和生物工程中的应用。我们进行了 hBM-MSCs 冻干的初步研究。将海藻糖负载到 hBM-MSCs 中。测量冻干悬浮液的玻璃化转变温度，为冷却和初级干燥实验提供信息。冻干后，测试了各种复水过程。hBM-MSCs 的最高回收率为（69.33±13.08）%。讨论了可能改善冻干结果的方法。总之，本研究为 hBM-MSCs 的冻干奠定了基础。
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本文引用的文献
1
Desiccation tolerance of prokaryotes: application of principles to human cells.原核生物的干燥耐受性：原理在人类细胞中的应用。
Integr Comp Biol. 2005 Nov;45(5):800-9. doi: 10.1093/icb/45.5.800.
2
An experimental study of the use of ultrasound to facilitate the loading of trehalose into platelets.利用超声促进海藻糖载入血小板的实验研究
Cryobiology. 2009 Oct;59(2):135-40. doi: 10.1016/j.cryobiol.2009.06.003. Epub 2009 Jun 16.
3
Bone marrow-derived human mesenchymal stem cells become quiescent on soft substrates but remain responsive to chemical or mechanical stimuli.源自骨髓的人间充质干细胞在柔软基质上会进入静止状态，但对化学或机械刺激仍保持反应能力。
Tissue Eng Part A. 2009 Jan;15(1):147-54. doi: 10.1089/ten.tea.2007.0388.
4
Evaluation of bone marrow mesenchymal stem cell standard cryopreservation procedure efficiency.骨髓间充质干细胞标准冷冻保存程序效率评估
Transplant Proc. 2008 Apr;40(3):839-41. doi: 10.1016/j.transproceed.2008.03.004.
5
Freeze-drying of human platelets: influence of saccharide, freezing rate and cell concentration.人血小板的冷冻干燥：糖类、冷冻速率和细胞浓度的影响
Cryo Letters. 2007 May-Jun;28(3):187-96.
6
Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells.冷冻保存的人骨髓间充质干细胞的体外扩增与多能分化
J Zhejiang Univ Sci B. 2007 Feb;8(2):136-46. doi: 10.1631/jzus.2007.B0136.
7
Constraints of tolerance: why are desiccation-tolerant organisms so small or rare?耐受性的限制：为何耐旱生物如此微小或罕见？
J Exp Biol. 2006 May;209(Pt 9):1575-84. doi: 10.1242/jeb.02179.
8
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Cryobiology. 2005 Oct;51(2):152-64. doi: 10.1016/j.cryobiol.2005.06.002.
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Morphology study of freeze-drying mononuclear cells of human cord blood.人脐带血冻存单个核细胞的形态学研究
Cryo Letters. 2005 May-Jun;26(3):193-200.
10
Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas.人骨髓间充质干细胞在胶质瘤治疗中的应用
Cancer Res. 2005 Apr 15;65(8):3307-18. doi: 10.1158/0008-5472.CAN-04-1874.
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