Verma Pooja, Jain Anju, Patra Surajeet Kumar, Gandhi Shipra, Sherwal B L, Chaudhary Monisha
Department of Biochemistry, Lady Hardinge Medical College and Associated Hospitals, New Delhi.
Indian J Tuberc. 2010 Jul;57(3):128-33.
Although pulmonary tuberculosis (PTB) is the most common manifestation of tuberculosis, extra pulmonary tuberculosis(EPTB) has equal significance. Among the extra pulmonary manifestations, tubercular lymphadenitis (TBL) is the most common form.
To perform PCR on fine needle aspirates of lymphnode by using hupB gene as target. To compare the sensitivity and specificity of PCR with culture, cytology, serology and clinical response to therapy.
MATERIAL & METHODS: After processing the samples by Universal Sample Processing(USP) method,two step nested PCR was performed using two sets of primers (N1S1 & CTFR) of hupB gene. All patients were put on ATT and were followed up for two months. The response to therapy was considered as the gold standard in our study.
The PCR assay for hupB gene was positive in 85 patients. Of these, 82% patients showed infection with M. tuberculosis, 1% was positive for M. bovis and 2% showed coinfection with both M. tuberculosis and M. bovis. The PCR assay of hupB gene in our study showed a sensitivity of 87.4% and specificity of 66.7%.
PCR assay for hup B gene is a rapid means of diagnosis of tubercular lymphadenitis.
尽管肺结核（PTB）是结核病最常见的表现形式，但肺外结核（EPTB）也具有同等重要性。在肺外表现中，结核性淋巴结炎（TBL）是最常见的形式。
以hupB基因为靶点，对淋巴结细针穿刺抽吸物进行聚合酶链反应（PCR）。比较PCR与培养、细胞学、血清学以及治疗临床反应的敏感性和特异性。
采用通用样本处理（USP）方法处理样本后，使用hupB基因的两组引物（N1S1和CTFR）进行两步巢式PCR。所有患者均接受抗结核治疗（ATT），并随访两个月。在我们的研究中，治疗反应被视为金标准。
85例患者的hupB基因PCR检测呈阳性。其中，82%的患者感染结核分枝杆菌，1%的患者牛分枝杆菌呈阳性，2%的患者同时感染结核分枝杆菌和牛分枝杆菌。我们研究中hupB基因的PCR检测显示敏感性为87.4%，特异性为66.7%。
hupB基因的PCR检测是诊断结核性淋巴结炎的快速方法。
