MRC Epidemiology Unit (South Wales), Llandough Hospital, Penarth, South Glamorgan, CF64 2XW.
Platelets. 1994;5(4):186-92. doi: 10.3109/09537109409006045.
Blood behaviour under shear-stress is assessed by a filter method in the Caerphilly Prospective Heart Study. Associations with haemostatic and lifestyle factors in 941 men aged 55 to 69 years are reported. The importance of platelets in filter blockage is confirmed, and roles for white cells and plasma viscosity in determining flow prior to blockage are identified. The aspect of platelet activity involved in blockage appears to be independent of mechanisms mediated by cyclo-oxygenase and adenosine diphosphate. von Willebrand factor however is associated with filter blockage, probably as an inter-platelet ligand. Fibrinogen is not required for blockage. Men regularly consuming alcohol or garlic supplements show reduced blockage, and biochemical mechanisms are suggested which might explain these associations. No effects of aspirin, smoking or consumption of fish oil supplements were noted. Shear-induced filter blockage is a simple method for measuring complex flow-dependent interactions of haemostatic factors. Its application in a wide range of investigations, including epidemiological studies, would appear to be appropriate.
在卡菲利前瞻性心脏研究中，采用过滤法评估切应力下的血液行为。报告了 941 名年龄在 55 至 69 岁的男性的止血和生活方式因素与过滤阻塞的相关性。证实了血小板在滤器阻塞中的重要性，并确定了白细胞和血浆粘度在阻塞前决定血流的作用。涉及阻塞的血小板活性方面似乎独立于由环氧化酶和二磷酸腺苷介导的机制。然而，血管性血友病因子与滤器阻塞相关，可能作为血小板间的配体。纤维蛋白原不是阻塞所必需的。经常饮酒或大蒜补充剂的男性显示阻塞减少，并提出了可能解释这些关联的生化机制。未观察到阿司匹林、吸烟或鱼油补充剂的影响。剪切诱导的滤器阻塞是一种测量止血因子复杂的依赖于流动的相互作用的简单方法。它在广泛的研究中的应用，包括流行病学研究，似乎是合适的。
